| 論文英文摘要 |
Botrytis cinerea is one of the most devastating fungal pathogens on many crops in Taiwan. Thirty-six single-conidium isolates collected from strawberry fields during 2018~2019 were investigated for fungicide sensitivity in this study. We evaluated the effective concentrations for 50% mycelial growth inhibition (EC_(50)) of four fungicides for each isolate and determined possible association with target site mutations in the cytb, β-tubulin, sdhB and CYP51 genes responsible for insensitivity to the fungicides pyraclostrobin (quinone outside inhibitor), carbendazim (benzimidazole), boscalid (succinate dehydrogenase inhibitor) and myclobutanil (sterol demethylation inhibitor), respectively. The results indicated that fungal insensitivities to the respective fungicides were highly associated with previously published genotypes, including G143A in cytb; E198A/V in β-tubulin; N230I and H272R/Y in sdhB; and Y136F in CYP51. Accordingly, molecular detection of fungicide insensitivity of B. cinerea is practicable. Furthermore, the numbers of isolates with insensitivity to pyraclostrobin, carbendazim, boscalid and myclobutanil were 31, 32, 25 and 1, respectively. The numbers of isolates with multifungicide insensitivities of two (pyraclostrobin and carbendazim) and three (plus boscalid) were 31 and 25, respectively. |