| 論文英文摘要 |
Botrytis cinerea is a prevalent and devastating fungal pathogen on strawberry in Taiwan. Thirty-six selected single-conidium isolates collected from strawberry fields islandwide were identified as B. cinerea sensu stricto based on B. cinerea-specific primer pair C729+/- and belonged to Genetic Group II based on PCRRFLP of the Bc-hch locus. The 36 isolates were all heterothallic isolates. Twenty-three isolates belonged to MAT1-1 type and 13 were characterized as MAT1-2 type. The four transposon genotypes, transposa, Boty-only, Flipper-only, and vacuma, comprised 55.5%, 38.9%, 0.0%, and 5.6%, respectively, of all the isolates studied. Combined analyses of mating types, transposon genotypes and single nucleotide polymorphisms of RPB2, HSP60 and G3PDH genes of B. cinerea isolates were performed to assess their genetic population structure and diversity. Genetic diversity indexes within Dahu-Shihtan population were all higher than those of the other areas population. Pairwise estimates of Gst, Nm and Nei's unbiased genetic identity and genetic distance between these two geographical populations revealed that low genetic differentiation and high gene flow existed between them. Hierarchical analysis of molecular variance of these two populations further indicated that 90.11% of the variation occurred within populations, while the variation between populations was only 9.89%. This is the first report of the genotypic diversity of B. cinerea populations on strawberry in Taiwan. |