||Fluopyram is a succinate dehydrogenase inhibitor (SDHI) fungicide that affects the synthesis of ATP and causes paralysis of nematodes. Fluopyram-based products are currently registered for many crops to mitigate root-knot nematodes. Although Aphelenchoides spp., which causes losses to rice, nest ferns, and orchids, are not listed as fluopyram-targeted nematodes, this genus has many economically important species in Taiwan. In this study, the mortality rates of three Meloidogyne spp., Rotylenchulus sp., and two Aphelenchoides spp. were recorded after treatments with 3 different concentrations of fluopyram. Meloidogyne spp. and A. besseyi all had a 100% mortality after being treated with different concentrations of fluopyram for 24 hr. However, A. bicaudatus had few nematodes survive. The Rotylenchulus sp. had mortality rates 37.9%, 30.3%, and 33.1% after treatments with the 1500X, 3000X, and 6000X fluopyram concentrations, respectively. Fluopyram was effective at killing Meloidogyne spp. and Aphelenchoides spp., but not Rotylenchulus sp., and a few A. bicaudatus individuals still survive. The results indicate that fluopyram sensitivity differs among different genera and species. For the field application, the nematodes' sensitivity to fluopyram, application methods, and phytotoxicity are factors that need to be considered. Further experiments are needed to confirm the feasibility of recommending fluopyram to control Aphelenchoides spp. on rice and other crops.